Fish High density lipoprotein (HDL) ELISA Kit Gene Bio Systems

Species Reactivity:Fish

UniProt:N/A

Abbreviation:HDL

Alternative Names:N/A

Application:ELISA

Range:Request Information

Sensitivity:Request Information

Intra-AssayCV:?6.2%

Inter-AssayCV:?98%

Recovery:0.97

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate HDL in samples. An antibody specific for HDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHDL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HDL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HDL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:High-density lipoproteins (HDL) form a class of lipoproteins, varying somewhat in their size (8–11 nm in diameter), that carry cholesterol from the bodys tissues to the liver. About thirty percent of blood cholesterol is carried by HDL.HDL is the smallest of the lipoprotein particles. They are the densest because they contain the highest proportion of protein.It is hypothesised that HDL can remove cholesterol from atheroma within arteries and transport it back to the liver for excretion or re-utilization—which is the main reason why HDL-bound cholesterol is sometimes called “good cholesterol”, or HDL-C. A high level of HDL-C seems to protect against cardiovascular diseases, and low HDL cholesterol levels (less than 40 mg/dL) increase the risk for heart disease. When measuring cholesterol, any contained in HDL particles is considered as protection to the bodys cardiovascular health, in contrast to “bad” LDL cholesterol.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).