Guinea pig Stem cell factor/mast cell growth factor (SCF) ELISA Kit Gene Bio Systems

Species Reactivity:Guinea pig (Cavia)

UniProt:N/A

Abbreviation:SCF

Alternative Names:MGF

Application:ELISA

Range:15.6-1000 pg/mL

Sensitivity:6.4 pg/mL

Intra-AssayCV:?4.2%

Inter-AssayCV:?7.9%

Recovery:0.9

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate SCF in samples. An antibody specific for SCF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:Stem cell factor (SCF;MGF) is a cytokine which binds CD117 (c-Kit). SCF is also known as “steel factor” or “c-kit ligand”. SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E(burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid). SCF, along with bFGF (basic fibroblast growth factor) and LIF (leukemia inhibitory factor), prevents spontaneous differentiation of primitive embryonic stem cells in cell culture.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).