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HiScript II One Step RT-PCR Kit Gene Bio Systems

Original price was: $ 269,00.Current price is: $ 107,60.

SKU: mode_6711942905956 Category: Uncategorized Tag: Reverse Transcription

Description
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Size: 125 rxn (20 uL/reaction)

Validation Results:

A. Taking total RNA of Hela cells as template, the GAPDH fragment of 555 bp was amplified using HiScript II One Step RT-PCR Kit (Vazyme, #P611). The detection sensitivity can reach 0.1 pg.

B. Taking PPRSV RNA as template, a fragment of 660 bp was amplified using HiScript II One Step RT-PCR Kit (Vazyme, #P611). The detection sensitivity can reach 0.2 TCLD50.

C. Taking 1 μg of total RNA from Hela cells as template, fragments with different lengths were amplified using HiScript II One Step RT-PCR Kit (Vazyme, #P611). The UTRN fragments as long as 12.1 kb were successfully amplified.

The Vazyme HiScript II One Step RT-PCR Kit is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains HiScript II Reverse Transcriptase, Champagne Taq plus hot-start DNA Polymerase, and an optimized buffer, which enables high-sensitive total RNA detection and long-fragment amplification (as long as 10 kb).

Components	P611-01 50 rxn (50 ul/rxn)
RNase free ddH₂O	1 ml×2
2 × One Step Mix^a	1 ml×2
One Step Enzyme Mix^b	125 μl
10 × Loading buffer	1.25 ml

a. Contains dNTPs.

b. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.

Storage: All components should be stored at -20°C.

Prompt Shipping and professional packaging

Because of our long-standing partnership in a long-standing partnership with UPS, FedEx, DHL and a variety of other leading global carriers, we can provide a variety of shipping options. Our warehouse staff are highly trained to package your goods exactly as per the specifications we offer. Your goods are thoroughly checked and securely secured prior to shipment. We deliver to thousands of customers every day from all over the world. This shows that we're committed to being the largest retailer online in the world. The warehouses and centers of distribution are situated in Europe, as well as the USA.

Note: Orders that contain more than one item are assigned a particular processing time, based on the individual item.

Prior to shipping We will inspect thoroughly the items you have ordered. The majority of orders will be sent within 48 hours. It is expected that delivery will take between three and seven days.

Returns

The inventory is always changing and we do not completely manage it due to the fact that several parties are involved, including our factory and warehouse. The actual stock levels can fluctuate at any moment. Be aware that it is possible that your order will run out of stock even after you've placed your order.

Our policy runs for 30 days. However, if 30 days have passed from the date you purchased the item, we cannot give you a refund or exchange.

To be eligible for a return, your product must be unopened and in the same condition as when you received it. The item must be in its original packaging.

HiScript II One Step RT-PCR Kit Gene Bio Systems

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