Size: 200 rxn (10 μl/rxn)
• Based on high effective HiScript II Reverse Transcriptase.
• High Stability: the recommended reaction temperature is 42℃-55℃.
• Anchored Oligo(dT)23VN: further improves the specificity, efficiency, and success rate of the first-strand cDNA synthesis.
• Ready-to-use SuperMix.
|
R211 |
R212 |
R222 |
R223 |
R232 |
R233 |
Application |
|
|
|
|
|
|
RT-qPCR |
|
|
√ |
√ |
√ |
√ |
universal |
√ |
√ |
|
|
|
|
Features |
|
|
|
|
|
|
Ready-to use |
|
|
√ |
√ |
√ |
√ |
Long cDNA synthesis |
√ |
√ |
|
|
√ |
√ |
gDNA wiper |
|
√ |
|
√ |
|
√ |
Primers |
|
|
|
|
|
|
Oligo (dT)23VN/N6 Mix |
|
|
√ |
√ |
|
|
self-selection |
√ |
√ |
|
|
√ |
√ |
Table 1. Guidelines for choice of HiScript II two-step RT-PCR/qPCR series.
The Vazyme HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR is specially designed for 2-step RT-qPCR. The 5× Mix contains all necessary components needed for reverse transcription, including Buffer, dNTPs, HiScript II Reverse Transcriptase, RNase inhibitor, and Random primers/Oligo-dT primer mix.
The HiScript II Q RT SuperMix for qPCR has been specially optimized for qPCR. For example, the ratio of Random primers/Oligo-dT primer is optimized to enable cDNA synthesis at any region of the template RNA and to ensure the repeatability of qPCR results. The cDNA products are compatible for SYBR- or probe-based qPCR, such as AceQ qPCR SYBR Green Master Mix (Vazyme, #Q111), ChamQ SYBR qPCR Master Mix (Vazyme, #Q311), ChamQ Color SYBR qPCR Master Mix (Vazyme, #Q411), and AceQ qPCR Probe Master Mix (Vazyme, #Q112).