Horse Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA Kit Gene Bio Systems

Species Reactivity:Horse (Equus caballus; Equine)

UniProt:Q3ZTK5

Abbreviation:TNF/TNFA/TNFSF8

Alternative Names:DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein|TNF superfamily; member 2|TNF; macrophage-derived|TNF; monocyte-derived|cachectin|tumor necrosis factor alpha

Application:ELISA

Range:7.8-500 pg/mL

Sensitivity:2.8 pg/mL

Intra-AssayCV:?5.5%

Inter-AssayCV:?8.9%

Recovery:1.06

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate TNF/TNFA/TNFSF8 in samples. An antibody specific for TNF/TNFA/TNFSF8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNF/TNFA/TNFSF8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF/TNFA/TNFSF8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF/TNFA/TNFSF8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-?is released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-?include B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).