Horse Vitamin K-dependent protein C (PROC) ELISA Kit Gene Bio Systems

Species Reactivity:Horse (Equus caballus; Equine)

UniProt:Q28380

Abbreviation:PROC

Alternative Names:APC; PC; PROC1; anticoagulant protein C|autoprothrombin IIA|blood coagulation factor XIV|protein C|vitamin K-dependent protein C

Application:ELISA

Range:Request Information

Sensitivity:Request Information

Intra-AssayCV:?3.9%

Inter-AssayCV:?7.6%

Recovery:1.01

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate PROC in samples. An antibody specific for PROC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:Protein C is a major physiological anticoagulant. It is a vitamin K-dependent serine protease enzyme (EC 3.4.21.69) that is activated by thrombin into activated protein C (APC). The activated form (with protein S and phospholipid as a cofactor) degrades Factor Va and Factor VIIIa. It should not be confused with C peptide or c-reactive protein or protein kinase C.The protein C pathway’s key enzyme, activated protein C, provides physiologic antithrombotic activity and exhibits both anti-inflammatory and anti-apoptotic activities. Its actions are related to development of thrombosis and ischemic stroke. The protein C pathway of the coagulation of the blood involves the influences of lipids and lipoproteins and the study of the strong epidemiologic association between hyperlipidemia and hypercoagulability.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).