Bird Influenza A virus subtype H5N1 IgG antibody (AIV-H5N1Ab-IgG) ELISA Kit Gene Bio Systems

Species Reactivity:Bird

UniProt:N/A

Abbreviation:AIV-H5N1Ab-IgG

Alternative Names:N/A

Application:ELISA

Range:Request Information

Sensitivity:Request Information

Intra-AssayCV:?6.8%

Inter-AssayCV:?8.5%

Recovery:0.91

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Competitive ELISA

Analysis Method??:Qualitative

Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AIV-H5N1Ab-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated AIV-H5N1Ab-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled AIV-H5N1Ab-IgG and unlabeled AIV-H5N1Ab-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of AIV-H5N1Ab-IgG in the sample. The color development is stopped and the intensity of the color is measured.

Product Overview:

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).