Chicken Tolloid-like protein 1 (TLL1) ELISA Kit Gene Bio Systems

Species Reactivity:Chicken (Gallus)

UniProt:Q9DER7

Abbreviation:TLL1

Alternative Names:ASD6; TLL;

Application:ELISA

Range:Request Information

Sensitivity:Request Information

Intra-AssayCV:?4.9%

Inter-AssayCV:?7.2%

Recovery:0.89

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate TLL1 in samples. An antibody specific for TLL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:TLL1 encodes an astacin-like zinc-dependent metalloprotease and is a subfamily member of the metzincin family. A similar protein in mice is required during heart development and specifically processes procollagen C-propeptides and chordin at similar cleavage sites. As previously demonstrated for BMP1 and TLD, TLL1 specifically processes procollagen C-propeptides at the physiologically relevant site, whereas TLL2 lacks this activity. BMP1 and TLL1 cleave chordin, at sites similar to procollagen C-propeptide cleavage sites, and counteract dorsalizing effects of chordin upon overexpression on Xenopus embryos. Proteases TLD and TLL2 do not cleave chordin.TLL1 gene plays multiple roles in formation of the mammalian heart and is essential for formation of the interventricular septum.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).