EasyTaq® DNA Polymerase Gene Bio Systems

EasyTaq^® DNA Polymerase

• • Product Details

    EasyTaq^® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq^® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq^® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.

    • Extension rate is about 1-2 kb/min.
    • Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY^®-T vectors.
    • Amplification of genomic DNA fragment up to 4 kb.

    Applications
    • Routine PCR
    • High throughput PCR
    • Colony PCR

    Storage
    at -20 ℃ for two years

    Product Contents

    Component	AP111-01	AP111-02	AP111-03	AP111-04
    EasyTaq® DNA Polymerase	500 U×1	500 U×6	2500 U×4	5000 U×10
    10×EasyTaq® Buffer	1.2 ml×1	1.2 ml×6	1.2 ml×20	1.2 ml×100
    6×DNA Loading Buffer	1 ml×1	1 ml×2	1 ml×4	1 ml×20

    Without dNTP, which is seperately available. 

    

    Citations

    Zhu C , Gao Y , Guo H , et al. Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution[J]. Cell Stem Cell, 2017, 20(5):720-731.

    Shao N , Jiang S , Zhang M , et al. MACRO: A Combined Microchip-PCR and Microarray System for High-throughput Monitoring of Genetically Modified Organisms[J]. Analytical Chemistry, 2013, 86(2):1269-1276.

    Li J , Fu J , Chen Y , et al. The U6, Biogenesis-Like 1, Plays an Important Role in Maize Kernel and Seedling Development by Affecting the 3′ End Processing of U6 snRNA[J]. Molecular Plant, 2017, 10(3):470-482.

    Simond AM , Rao T , Zuo D , et al. ErbB2-positive mammary tumors can escape PI3K-p110α loss through downregulation of the Pten tumor suppressor[J]. Oncogene, 2017, 36(43):6059-6066.

    Li Y , Guo SJ , Shao N , et al. A universal multiplex PCR strategy for 100-plex amplification using a hydrophobically patterned microarray[J]. LAB ON A CHIP, 2011, 11(21):3609-8.