Size :100ul
Clone Number:
Aliases:4E-BP1 antibody; 4EBP1 antibody; 4EBP1_HUMAN antibody; BP 1 antibody; eIF4E binding protein 1 antibody; eIF4E-binding protein 1 antibody; Eif4ebp1 antibody; Eukaryotic translation initiation factor 4E-binding protein 1 antibody; PHAS-I antibody; PHASI antibody; Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
Product Type:Polyclonal Antibody
Immunogen Species:Homo sapiens (Human)
UniProt ID:Q13541
Immunogen:Peptide sequence around aa.34~38 (S-T-T-P-G) derived from Human 4E-BP1.
Raised in:Rabbit
Species Reactivity:Human, Mouse, Rat
Tested Applications:ELISA, WB, IHC; WB:1:500-1:1000, IHC:1:50-1:200
Background:4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Gingras AC, et al. Genes Dev Nov. 2005 Feb 15.
Mothe-Satney I, et al.(2000) J Biol Chem:15(21): 2852-64.
Gingras AC, et al. (1999) Genes Dev:13(11): 1422-37.
Lal L, et al.(2005) Blood;105(4): 1669-7.
Clonality:Polyclonal
Isotype:IgG
Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Conjugate:Non-conjugated
Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form:liquid
Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names:EIF4EBP1
Research Areas:Epigenetics and Nuclear Signaling;Metabolism