ESR1 Monoclonal Antibody – Cat. #: CSB-MA442659 Gene Bio Systems

Size :50ul

Clone Number:

Aliases:Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1), ESR1, ESR NR3A1

Product Type:Monoclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P03372

Immunogen:Synthesized peptide derived from human Estrogen Receptor ?

Raised in:Mouse

Species Reactivity:Human

Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Background:Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1.

Clonality:Monoclonal

Isotype:IgG2b, Kappa

Purification Method:The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Conjugate:Non-conjugated

Buffer:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form:Liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:ESR1

Research Areas:Signal Transduction