Bovine Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit Gene Bio Systems

Species Reactivity:Bovine (Bos taurus; Cattle)

UniProt:A6H7B1

Abbreviation:TOMM20

Alternative Names:RP4-597N16.2; KIAA0016; MAS20; MGC117367; MOM19; TOM20; translocase of outer mitochondrial membrane 20 homolog|translocase of outer mitochondrial membrane 20 homolog type II

Application:ELISA

Range:Request Information

Sensitivity:Request Information

Intra-AssayCV:?6.8%

Inter-AssayCV:?7.2%

Recovery:1.03

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate TOMM20 in samples. An antibody specific for TOMM20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:The protein import machinery of the mitochondrial outer membrane is comprised of a dynamic complex of proteins that mediates translocation of cytosolic precursor proteins into or across the membrane. Several proteins within this complex have been identified, such as Mas20p in yeast and MOM19 in Neurospora crassa .
The 145-amino acid human polypeptide shares high similarity with Mas20p and MOM19 within the N-terminal region, but exhibits only weak homology to the tetratricopeptide-repeat B domain that is found in the other 2 proteins. The authors showed that human Mas20p is targeted and inserted into the outer membrane of isolated rat heart mitochondria in the N(in)-to-C(cyto) orientation.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).