Bovine Prolactin-releasing peptide receptor (PRLHR) ELISA Kit Gene Bio Systems

Species Reactivity:Bovine (Bos taurus; Cattle)

UniProt:Q4EW11

Abbreviation:PRLHR

Alternative Names:GPR10; GR3; MGC126539; MGC126541; PrRPR; G protein-coupled receptor 10|OTTHUMP00000020584|prolactin releasing peptide receptor|prolactin-releasing hormone receptor

Application:ELISA

Range:Request Information

Sensitivity:Request Information

Intra-AssayCV:?5.6%

Inter-AssayCV:?7.3%

Recovery:0.92

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate PRLHR in samples. An antibody specific for PRLHR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRLHR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRLHR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRLHR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:The prolactin-releasing hormone receptor (PRRP) is a G-protein coupled receptor that binds the prolactin releasing hormone.PRLHR is a 7-transmembrane domain receptor for prolactin-releasing hormone that is highly expressed in anterior pituitary
By screening a placenta genomic DNA library, followed by 5-prime and 3-prime RACE of pituitary and adenoma cDNA libraries, Ozawa et al. (2002) isolated a full-length cDNA encoding PRLHR, which they called PRRPR. They identified multiple transcription start sites. The predicted 370-amino acid protein contains 7 hydrophobic regions. Northern blot analysis revealed expression of a 2.2-kb transcript in pituitary adenomas.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).