Bovine Secretory phospholipase A2 receptor (PLA2R1) ELISA Kit Gene Bio Systems

Species Reactivity:Bovine (Bos taurus; Cattle)

UniProt:P49259

Abbreviation:PLA2R1

Alternative Names:CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R; phospholipase A2 receptor 1

Application:ELISA

Range:7.81-500 pg/mL

Sensitivity:1.95 pg/mL

Intra-AssayCV:?3.7%

Inter-AssayCV:?6.9%

Recovery:0.99

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate PLA2R1 in samples. An antibody specific for PLA2R1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2R1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2R1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2R1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:Sequence analysis revealed that the deduced 1,465-amino acid PLA2R1 transmembrane protein contains a 25-amino acid signal sequence, 15 potential N-glycosylation sites, an N-terminal cysteine-rich domain, a fibronectin-like type II domain, 8 carbohydrate recognition domains, and a short C-terminal cytoplasmic tail containing a consensus sequence (asn-pro-X-tyr) also found in bovine Pla2r1. A casein-kinase II phosphorylation site is also located in this C-terminal region. Ancian et al. (1995) identified a splice variant encoding a soluble form of PLA2R1. Quantitative PCR analysis suggested that the ratio for the transcription of membrane to soluble PLA2R1 is 1.6. Northern blot analysis detected strong expression of 6.5- and 5.4-kb PLA2R1 transcripts in kidney, with moderate expression in placenta, lung, and skeletal muscle.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).