Bovine Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (PPP2CA) ELISA Kit Gene Bio Systems

Species Reactivity:Bovine (Bos taurus; Cattle)

UniProt:P67774

Abbreviation:PPP2CA

Alternative Names:PP2Ac; PP2CA; PP2Calpha; RP-C; PP2A-alpha|protein phosphatase 2; catalytic subunit; alpha isoform|replication protein C|serine/threonine protein phosphatase 2A; catalytic subunit; alpha isoform|seri

Application:ELISA

Range:31.25-2000 pg/mL

Sensitivity:7.81 pg/mL

Intra-AssayCV:?6.3%

Inter-AssayCV:?10.8%

Recovery:1.07

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate PPP2CA in samples. An antibody specific for PPP2CA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP2CA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP2CA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP2CA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:PPP2Ca encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The mammalian enzyme can be isolated as a catalytic subunit of 36 kD complexed to 1 regulatory subunit of 65 kD and to another regulatory subunit of varying molecular mass, depending on the tissue and the separation technique used. Two isoforms of the catalytic subunit of PP2A, alpha and beta, are demonstrable in many mammalian species.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).