Bovine Troponin T, slow skeletal muscle (TNNT1) ELISA Kit Gene Bio Systems

Species Reactivity:Bovine (Bos taurus; Cattle)

UniProt:Q8MKH6

Abbreviation:TNNT1

Alternative Names:ANM; FLJ98147; MGC104241; STNT; TNT; TNTS; slow skeletal muscle troponin T|troponin T1; skeletal; slow|troponin-T1; skeletal; slow

Application:ELISA

Range:Request Information

Sensitivity:Request Information

Intra-AssayCV:?4.7%

Inter-AssayCV:?8.4%

Recovery:0.92

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate TNNT1 in samples. An antibody specific for TNNT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:TNNT1 is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).