Canine Interleukin 4 (IL-4) ELISA Kit Gene Bio Systems

Species Reactivity:Canine (Canis familiaris; Dog)

UniProt:O77762

Abbreviation:IL4

Alternative Names:BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1|B_cell stimulatory factor 1|lymphocyte stimulatory factor 1

Application:ELISA

Range:7.8-500 pg/mL

Sensitivity:3.9 pg/mL

Intra-AssayCV:?7.2%

Inter-AssayCV:?9.6%

Recovery:0.8

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate IL4 in samples. An antibody specific for IL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).