Canine Natriuretic peptides A (NPPA) ELISA Kit Gene Bio Systems

Species Reactivity:Canine (Canis familiaris; Dog)

UniProt:P07499

Abbreviation:NPPA

Alternative Names:ANF; ANP; ATFB6; CDD-ANF; PND; atrial natriuretic factor|atriopeptin|cardionatrin|prepronatriodilatin

Application:ELISA

Range:15.6-1000 pg/mL

Sensitivity:7.7 pg/mL

Intra-AssayCV:?6.2%

Inter-AssayCV:?8.7%

Recovery:1

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate NPPA in samples. An antibody specific for NPPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNPPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPPA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).