Fish Heat Shock Protein 70 (HSP-70) ELISA Kit Gene Bio Systems

Species Reactivity:Fish

UniProt:N/A

Abbreviation:HSP-70

Alternative Names:N/A

Application:ELISA

Range:0.625-10 ng/mL

Sensitivity:0.31 ng/mL

Intra-AssayCV:?5.1%

Inter-AssayCV:?9.4%

Recovery:1.01

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate HSP-70 in samples. An antibody specific for HSP-70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHSP-70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP-70 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP-70 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 – 8°C, which means 7 days at 37°C equaling 12 months at 2 – 8°C).