FlyCut® BamHI is expressed and purified from E.coli that carries the recombinant BamHI gene. The molecular weight is 27.5 kDa, with the recognition site at G^GATCC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’…G↓GATCC…3’
3’…CCTAG↑G…5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)