Size:100ug
Purification:protein A+G purified
Form:liquid
Purity:?95% as determined by SDS-PAGE
Host:Mouse
Clonality:monoclonal
Clone ID:5H12
Isotype:IgG1
Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background:Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).
Immunogen:hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
Synonyms:BHLHE78, MOP1, PASD8
Observed MW:120 kDa
Uniprot ID:Q16665
Reactivity:Human
Tested Application:ELISA, IF, WB
Recommended dilution:WB: 1:1000-1:10000; IF: 1:50-1:200
Gene ID:3091
Research Area:Signal Transduction, Metabolism, Cardiovascular, Developmental biology, Neuroscience, Stem Cells