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HiPure DNA/RNA/Protein KitR5211

Introduction

Hipure DNA / RNA / protein kit is the most rapid and simple method to simultaneously extract DNA, RNA and protein from cultured cells and animal soft tissue samples. This kit adopts column technology which is no need for dangerous phenol chloroform extraction and time-consuming alcohol precipitation in the extraction process. This kit is suitable for simultaneous extraction of RNA, DNA and protein from < 1x 10^{^}7 eukaryotic cultured cells as well as from animal soft tissues less than 20mg such asliver, kidney, spleen and brain. The purified RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection, in vitro translation while the obtained DNA can be directly used in PCR and Southern hybridization, the obtained protein can be directly used in SDS-PAGE electrophoresis, western blot and so on.

Details

Specifications

Principle

Advantages

• High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
• Fast – column method can complete the extraction of several samples in 30 minutes
• Safe – no phenol chloroform extraction required
• Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

Kit Contents

Storage and Stability

Hipure DNA/RNA/protein kit can be stored at room temperature (15-25℃) in dry for 18 months. For long-term storage, it shall be stored at 2-8℃. At low temperature, buffer RL may form precipitation. It needs 55℃ water bath to completely dissolve the precipitation before use. As DEPC treated water does not contain any bacteriostatic factors, it may be contaminated by bacterial or fungal when placed or operated at room temperature. We recommend to pack DEPC treated water separately and store it at -20℃ to reduce pollution. If DEPC treated water is polluted, please prepare it again.