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HiPure Fastfilter Plasmid Midi Kit GeneBio Systems

Name: Check out our website for the most recent HiPure Fastfilter Plasmid Midi Kit GeneBio Systems . Unique Designs you won't find elsewhere
SKU: mode_7123386269796
Price: 104.70 USD
Availability: InStock

Original price was: $ 349,00.Current price is: $ 104,70.

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SKU: mode_7123386269796 Category: Uncategorized

The HiPure Fastfilter Plasmid DNA Midi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes aspecial filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 250 µg high copy number plasmid DNA or 10-75 µg low copy number plasmid DNA can be purified from 15-50 mL overnight culture.

Specifications

Features	Specifications
Main Functions	Isolation up to 250μg plasmid DNA from 30-50ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling, etc.
Purification method	Midi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	30-50ml
Yield	50-250µg
Elution volume	≥300μl
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	250µg

Kit Contents

Contents	P101302	P101303
Purification Times	25 Preps	100 Preps
RNase A	10 mg	40 mg
Buffer P1	80 ml	300 ml
Buffer P2	80 ml	300 ml
Buffer LEN3	40 ml	150 ml
Buffer GBT	60 ml	250 ml
Buffer PW1	60 ml	250 ml
Buffer PW2	20 ml	100 ml
Elution Buffer	30 ml	120 ml
HiPure DNA Midi Columns III	25	100
Lysate Clear Midi Syringe	25	100
15 ml Collection Tubes	50	200

Advantages

High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – it takes only 15-60 minutes to complete the isolation
High yield – up to 70µg plasmid can be binded in one column

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Size	25 Preps

Prompt Shipping and professional packaging

Because of our long-standing partnership in a long-standing partnership with UPS, FedEx, DHL and a variety of other leading global carriers, we can provide a variety of shipping options. Our warehouse staff are highly trained to package your goods exactly as per the specifications we offer. Your goods are thoroughly checked and securely secured prior to shipment. We deliver to thousands of customers every day from all over the world. This shows that we're committed to being the largest retailer online in the world. The warehouses and centers of distribution are situated in Europe, as well as the USA.

Note: Orders that contain more than one item are assigned a particular processing time, based on the individual item.

Prior to shipping We will inspect thoroughly the items you have ordered. The majority of orders will be sent within 48 hours. It is expected that delivery will take between three and seven days.

Returns

The inventory is always changing and we do not completely manage it due to the fact that several parties are involved, including our factory and warehouse. The actual stock levels can fluctuate at any moment. Be aware that it is possible that your order will run out of stock even after you've placed your order.

Our policy runs for 30 days. However, if 30 days have passed from the date you purchased the item, we cannot give you a refund or exchange.

To be eligible for a return, your product must be unopened and in the same condition as when you received it. The item must be in its original packaging.

HiPure Fastfilter Plasmid Midi Kit GeneBio Systems

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