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Histone H3K27me3 Antibody – Cat. #: CSB-PA963638 Gene Bio Systems

Original price was: $ 1.003,60.Current price is: $ 250,90.

SKU: mode_6912255230052 Category: Uncategorized Tag: PRIMARY ANTIBODIES

Description
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Size :100ul

Clone Number:

Aliases:HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:GeneID:8290SwissProt:Q16695

Immunogen:A synthetic methylated peptide corresponding to residues surrounding K27 of Human histone H3

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB, IHC, IF, IP, ChIP; WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200

Background:

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.

2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.

3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.

4. Cheung, P. et al. (2000) Cell 103, 263-71.

5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.

6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.

7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.

8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.

9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.

10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.

11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antigen Affinity Purified

Conjugate:Non-conjugated

Buffer:Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:HIST3H3

Research Areas:Epigenetics and Nuclear Signaling

Prompt Shipping and professional packaging

Because of our long-standing partnership in a long-standing partnership with UPS, FedEx, DHL and a variety of other leading global carriers, we can provide a variety of shipping options. Our warehouse staff are highly trained to package your goods exactly as per the specifications we offer. Your goods are thoroughly checked and securely secured prior to shipment. We deliver to thousands of customers every day from all over the world. This shows that we're committed to being the largest retailer online in the world. The warehouses and centers of distribution are situated in Europe, as well as the USA.

Note: Orders that contain more than one item are assigned a particular processing time, based on the individual item.

Prior to shipping We will inspect thoroughly the items you have ordered. The majority of orders will be sent within 48 hours. It is expected that delivery will take between three and seven days.

Returns

The inventory is always changing and we do not completely manage it due to the fact that several parties are involved, including our factory and warehouse. The actual stock levels can fluctuate at any moment. Be aware that it is possible that your order will run out of stock even after you've placed your order.

Our policy runs for 30 days. However, if 30 days have passed from the date you purchased the item, we cannot give you a refund or exchange.

To be eligible for a return, your product must be unopened and in the same condition as when you received it. The item must be in its original packaging.

Histone H3K27me3 Antibody – Cat. #: CSB-PA963638 Gene Bio Systems

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